Manchester United have been drawn against Danish champions Midtjylland in the Europa League round of 32, while Liverpool will play Augsburg.
Louis van Gaal's side dropped into the Europa League after failing to reach the knockout phase of the Champions League.
Gary Neville's Valencia face Austrian side Rapid Wien, while Mauricio Pochettino's Tottenham Hotspur are in action against Fiorentina.
Bundesliga giants Borussia Dortmund take on Porto, who won the Europa League in 2011, while Bayer Leverkusen will go head-to-head with Sporting CP.
Sevilla, who are the current holders of the Europa League after retaining their title last season, face Norwegian outfit Molde.
Swiss club Basel, whose St. Jakob-Park stadium will host the final on May 18, will take on Ligue 1 team St-Etienne.
Round of 32. Ties to be played Feb. 18 and 25:
Valencia v Rapid Wien
Fiorentina v Tottenham
Borussia Dortmund v Porto
Fenerbahce v Lokomotiv Moscow
Anderlecht v Olympiakos
Midtjylland v Manchester United
Augsburg v Liverpool
Sparta Praha v Krasnodar
Galatasaray v Lazio
Sion v Braga
Shakhtar Donetsk v Schalke
Marseille v Athletic Bilbao
Sevilla v Molde
Sporting CP v Leverkusen
Villarreal v Napoli
St-Etienne v Basel