Manchester City captain Vincent Kompany has lost his battle to be fit for his side's decisive Champions League quarter-final clash against Paris Saint-Germain.
The influential Belgium defender had been hoping to feature in the second leg at the Etihad Stadium after a month on the sidelines with the latest in a long line of calf-related problems.
But despite the 30-year-old being involved in training, Kompany's involvement has been deemed too much of a risk by manager Manuel Pellegrini.
Speaking at his pre-match press conference, Pellegrini said: "Vincent Kompany is not 100 per cent."
Asked if he could still play, Pellegrini said: "No, it is not possible."
Pellegrini had previously suggested Kompany could be fit, so the news will come as a blow for a tie in which the score is locked at 2-2 after a thrilling first leg last week.
But City will have Nicolas Otamendi in the centre of defence despite a minor scare over a twisted ankle in the weekend win over West Brom and Pellegrini has faith in his back line.
Pellegrini said: "I am absolutely confident because with this defence we played a lot of games here in the Premier League and in the Champions League and if we are in this stage it is because we have good defenders.
"We have had a lot of injuries (this season) but I trust 100 per cent in the players who can play."
Playmaker David Silva will return after resting an ankle injury and the blow to the foot Sergio Aguero took in the latter stages on Saturday has not proved serious.
Pellegrini recognises both sides have everything to play for after an evenly-contested first encounter at the Parc des Princes in which both sides showed good some attacking play but also made costly errors.
The Chilean said: "When we started this stage against Paris Saint-Germain I said I didn't think one team could be favourites. I continue thinking exactly the same.
"We have to play a tough game against a very good team. We knew that before we played in Paris and we went there to try to win from the beginning.
"Tomorrow we are going to do exactly the same thing because it is the way this team play."
Laurent Blanc, meanwhile, has warned City that Zlatan Ibrahimovic is determined to shoot them down.
The prolific Ibrahimovic is the chief obstacle standing in City's way as they bid to reach the Champions League semi-finals.
Ibrahimovic had a mixed night in the first leg of their quarter-final in France last week, scoring and hitting the bar but also missing a penalty and spurning another good chance in the draw.
The 34-year-old has had a fine season, netting 30 goals in Ligue 1 for the runaway champions. He is out of contract at the end of the campaign and has been linked with a move to England, most recently with City's rivals Manchester United.
And boss Blanc expects him to be at his sharpest at the Etihad Stadium.
He said: "We're always a bit surprised when Zlatan misses a chance but when you're a top goalscorer like him, you can miss a couple.
"Unfortunately for us he missed them in that game. He still scored though and he's someone who is extremely demanding of himself.
"He wants to show something else tomorrow, he wants to be more clinical in front of goal. If we create as many chances as the first leg, I think his conversion rate will be a lot higher."
Blanc, who could have midfielder Marco Verratti back after almost eight weeks out with a groin injury, knows improvements are necessary if his side are to progress.
He said: "We've analysed the first game and looked at the errors we made. There was too wide a gap between players, we were not compact enough and opened too much space for City.
"When you think of the palyers they've got, particularly going forward, they can create problems for a lot of teams, not just us.
"We need to perform at the highest possible level, a higher level than the first game. PSG are capable of qualifying but we know how difficult it will be."